Premium
High‐Throughput Measurement of Binding Kinetics by mRNA Display and Next‐Generation Sequencing
Author(s) -
JalaliYazdi Farzad,
Huong Lai Lan,
Takahashi Terry T.,
Roberts Richard W.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201600077
Subject(s) - throughput , kinetics , chemistry , high throughput screening , computational biology , computer science , biology , biochemistry , physics , telecommunications , quantum mechanics , wireless
There is great demand for high‐throughput methods to characterize ligand affinity. By combining mRNA display with next‐generation sequencing, we determined the kinetic on‐ and off‐rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.