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Glycation Reactivity of a Quorum‐Sensing Signaling Molecule
Author(s) -
Tsuchikama Kyoji,
Gooyit Major,
Harris Tyler L.,
Zhu Jie,
Globisch Daniel,
Kaufmann Gunnar F.,
Janda Kim D.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201511911
Subject(s) - glycation , quorum sensing , chemistry , biochemistry , cell signaling , signal transduction , function (biology) , computational biology , microbiology and biotechnology , biology , receptor , virulence , gene
Reported herein is that (4 S )‐4,5‐dihydroxy‐2,3‐pentanedione (DPD) can undergo a previously undocumented non‐enzymatic glycation reaction. Incubation of DPD with viral DNA or the antibiotic gramicidin S resulted in significant biochemical alterations. A protein‐labeling method was consequently developed that facilitated the identification of unrecognized glycation targets of DPD in a prokaryotic system. These results open new avenues toward tracking and understanding the fate and function of the elusive quorum‐sensing signaling molecule.