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Development of a Two‐Photon Fluorescent Probe for Imaging of Endogenous Formaldehyde in Living Tissues
Author(s) -
Tang Yonghe,
Kong Xiuqi,
Xu An,
Dong Baoli,
Lin Weiying
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201510373
Subject(s) - fluorescence , endogeny , sodium bisulfite , formaldehyde , context (archaeology) , chemistry , living systems , biophysics , biochemistry , biology , optics , organic chemistry , physics , ecology , paleontology
Investigation of the physiological and pathological functions of formaldehyde (FA) are largely restricted by a lack of useful FA imaging agents, in particular, those that allow detection of FA in the context of living tissues. Herein, we present the rational design, synthesis, and photophysical property studies of the first two‐photon fluorescent FA probe, Na‐FA . Importantly, the highly desirable attributes of the probe Na‐FA (such as a very large turn‐on signal (up to 900‐fold), a low detection limit, and a very fast onset imparted by the unique design aspects of the probe), make it possible to monitor endogenous FA in living tissues for the first time. Furthermore, sodium bisulfite was identified as a simple and convenient inhibitor of FA within biological environments.