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A Catalytic DNA Activated by a Specific Strain of Bacterial Pathogen
Author(s) -
Shen Zhifa,
Wu Zaisheng,
Chang Dingran,
Zhang Wenqing,
Tram Kha,
Lee Christine,
Kim Peter,
Salena Bruno J.,
Li Yingfu
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201510125
Subject(s) - deoxyribozyme , strain (injury) , nucleic acid , dna , biology , clostridium difficile , microbiology and biotechnology , bacteria , pathogen , chemistry , computational biology , genetics , anatomy , antibiotics
Abstract Pathogenic strains of bacteria are known to cause various infectious diseases and there is a growing demand for molecular probes that can selectively recognize them. Here we report a special DNAzyme (catalytic DNA), RFD‐CD1, that shows exquisite specificity for a pathogenic strain of Clostridium difficile ( C. difficile ). RFD‐CD1 was derived by an in vitro selection approach where a random‐sequence DNA library was allowed to react with an unpurified molecular mixture derived from this strain of C. difficle , coupled with a subtractive selection strategy to eliminate cross‐reactivities to unintended C. difficile strains and other bacteria species. RFD‐CD1 is activated by a truncated version of TcdC, a transcription factor, that is unique to the targeted strain of C. difficle . Our study demonstrates for the first time that in vitro selection offers an effective approach for deriving functional nucleic acid probes that are capable of achieving strain‐specific recognition of bacterial pathogens.