Premium
A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells
Author(s) -
Wang Lu,
Yuan Lin,
Zeng Xian,
Peng Juanjuan,
Ni Yong,
Er Jun Cheng,
Xu Wang,
Agrawalla Bikram Keshari,
Su Dongdong,
Kim Beomsue,
Chang YoungTae
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201510003
Subject(s) - adenosine triphosphate , mitochondrion , atp–adp translocase , chemiosmosis , atp synthase , fluorescence , biophysics , intracellular , v atpase , microbiology and biotechnology , chemistry , membrane potential , biochemistry , inner mitochondrial membrane , biology , atpase , enzyme , physics , quantum mechanics
Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite‐binding switchable fluorescent probe, ATP‐Red 1 , which selectively and rapidly responds to intracellular concentrations of ATP. Live‐cell imaging indicated that ATP‐Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP‐Red 1 , we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell apoptosis. These results indicate that ATP‐Red 1 is a useful tool for investigating ATP‐relevant biological processes.