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Temporal Control of Membrane Fusion through Photolabile PEGylation of Liposome Membranes
Author(s) -
Kong Li,
Askes Sven H. C.,
Bonnet Sylvestre,
Kros Alexander,
Campbell Frederick
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201509673
Subject(s) - liposome , membrane , pegylation , biophysics , lipid bilayer fusion , chemistry , fusion , synthetic membrane , biochemistry , biology , polyethylene glycol , linguistics , philosophy
Membrane fusion results in the transport and mixing of (bio)molecules across otherwise impermeable barriers. In this communication, we describe the temporal control of targeted liposome–liposome membrane fusion and contents mixing using light as an external trigger. Our method relies on steric shielding and rapid, photoinduced deshielding of complementary fusogenic peptides tethered to opposing liposomal membranes. In an analogous approach, we were also able to demonstrate precise spatiotemporal control of liposome accumulation at cellular membranes in vitro.