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Combining Metabolic Engineering and Electrocatalysis: Application to the Production of Polyamides from Sugar
Author(s) -
Suastegui Miguel,
Matthiesen John E.,
Carraher Jack M.,
Hernandez Nacu,
Rodriguez Quiroz Natalia,
Okerlund Adam,
Cochran Eric W.,
Shao Zengyi,
Tessonnier JeanPhilippe
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201509653
Subject(s) - hexamethylenediamine , chemistry , muconic acid , adipic acid , metabolic engineering , electrocatalyst , vinasse , citric acid , biomass (ecology) , sugar , organic chemistry , polyamide , fermentation , oceanography , electrode , electrochemistry , enzyme , geology , benzene
Biorefineries aim to convert biomass into a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion, it is crucial to streamline the catalytic and downstream processing steps. In this work, a route that combines bio‐ and electrocatalysis to convert glucose into bio‐based unsaturated nylon‐6,6 is reported. An engineered strain of Saccharomyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic acid, with the highest reported muconic acid titer of 559.5 mg L −1 in yeast. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3‐hexenedioic acid in 94 % yield despite the presence of biogenic impurities. Bio‐based unsaturated nylon‐6,6 (unsaturated polyamide‐6,6) was finally obtained by polymerization of 3‐hexenedioic acid with hexamethylenediamine.