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Design of a Hyperpolarized Molecular Probe for Detection of Aminopeptidase N Activity
Author(s) -
Hata Ryunosuke,
aka Hiroshi,
Takakusagi Yoichi,
Ichikawa Kazuhiro,
Sando Shinsuke
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201509457
Subject(s) - chemistry , aminopeptidase , nuclear magnetic resonance spectroscopy , angiogenesis , reactivity (psychology) , enzyme , biochemistry , stereochemistry , cancer research , amino acid , biology , medicine , pathology , leucine , alternative medicine
Aminopeptidase N (APN) is an important enzyme that is involved in tumor angiogenesis. Detection of APN activity can thus lead to early diagnosis and elucidation of tumor development. Although some molecular probes for APN have been developed, the detection of APN activity in opaque biological samples remains a challenge. To this end, we designed a hyperpolarized NMR probe [1‐ 13 C]Ala‐NH 2 which satisfies the prerequisites for APN detection, namely, sufficient retention of the hyperpolarized state, a high reactivity to APN, and an APN‐induced chemical shift change. The [1‐ 13 C]Ala‐NH 2 probe allowed sensitive detection of APN activity using 13 C NMR spectroscopy.