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Target‐Induced and Equipment‐Free DNA Amplification with a Simple Paper Device
Author(s) -
Liu Meng,
Hui Christy Y.,
Zhang Qiang,
Gu Jimmy,
Kannan Balamurali,
JahanshahiAnbuhi Sana,
Filipe Carlos D. M.,
Brennan John D.,
Li Yingfu
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201509389
Subject(s) - pullulan , amplicon , dna , rolling circle replication , nanotechnology , reagent , chemistry , combinatorial chemistry , molecule , materials science , biophysics , polymerase chain reaction , biochemistry , biology , organic chemistry , dna replication , polysaccharide , gene
We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA‐enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.