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N‐Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent
Author(s) -
De Castro Cristina,
Speciale Immacolata,
Duncan Garry,
Dunigan David D.,
Agarkova Irina,
Lanzetta Rosa,
Sturiale Luisa,
Palmigiano Angelo,
Garozzo Domenico,
Molinaro Antonio,
Tonetti Michela,
Van Etten James L.
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201509150
Subject(s) - fucose , glycan , chemistry , rhamnose , residue (chemistry) , asparagine , glycosylation , xylose , stereochemistry , galactose , biochemistry , glycoprotein , amino acid , fermentation
N‐glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N‐glycan is herein described. This N‐glycan core structure, common to all chloroviruses, is a pentasaccharide with a β‐glucose linked to an asparagine residue which is not located in the typical sequon N‐X‐T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus‐dependent. Additional decorations occur on this core N‐glycan and represent a molecular signature for each chlorovirus.