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Breakable Hybrid Organosilica Nanocapsules for Protein Delivery
Author(s) -
Prasetyanto Eko Adi,
Bertucci Alessandro,
Septiadi Dedy,
Corradini Roberto,
CastroHartmann Pablo,
De Cola Luisa
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201508288
Subject(s) - nanocapsules , chemistry , nanotechnology , biomolecule , drug delivery , intracellular , biopharmaceutical , biophysics , biochemistry , materials science , nanoparticle , biology , microbiology and biotechnology
Abstract The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics to genetic engineering. However, the process mostly involves tedious techniques and often requires extensive alteration of the protein itself. Herein we report a straightforward approach to encapsulate native proteins by using breakable organosilica matrices that disintegrate upon exposure to a chemical stimulus. The biomolecule‐containing capsules were tested for the intracellular delivery of highly cytotoxic proteins into C6 glioma cells. We demonstrate that the shell is broken, the release of the active proteins occurs, and therefore our hybrid architecture is a promising strategy to deliver fragile biomacromolecules into living organisms.