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Spectra Library: An Assumption‐Free In Situ Method to Access the Kinetics of Catechols Binding to Colloidal ZnO Quantum Dots
Author(s) -
Lin Wei,
Haderlein Michael,
Walter Johannes,
Peukert Wolfgang,
Segets Doris
Publication year - 2016
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201508252
Subject(s) - ligand (biochemistry) , kinetics , colloid , spectral line , nanoparticle , quantum dot , chemistry , adsorption , colloidal gold , in situ , particle (ecology) , absorbance , nanotechnology , chemical physics , materials science , organic chemistry , physics , chromatography , quantum mechanics , astronomy , biochemistry , receptor , oceanography , geology
Assumption‐free and in situ resolving of the kinetics of ligand binding to colloidal nanoparticles (NPs) with high time resolution is still a challenge in NP research. A unique concept of using spectra library and stopped‐flow together with a “search best‐match” Matlab algorithm to access the kinetics of ligand binding in colloidal systems is reported. Instead of deconvoluting superimposed spectra using assumptions, species absorbance contributions (ligand@ZnO NPs and ligand in solution) are obtained by offline experiments. Therefrom, a library of well‐defined targets with known ligand distribution between particle surface and solution is created. Finally, the evolution of bound ligand is derived by comparing in situ spectra recorded by stopped‐flow and the library spectra with the algorithm. Our concept is a widely applicable strategy for kinetic studies of ligand adsorption to colloidal NPs and a big step towards deep understanding of surface functionalization kinetics.