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Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats
Author(s) -
Kojima Ryosuke,
Takakura Hideo,
Kamiya Mako,
Kobayashi Eiji,
Komatsu Toru,
Ueno Tasuku,
Terai Takuya,
Hanaoka Kenjiro,
Nagano Tetsuo,
Urano Yasuteru
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201507530
Subject(s) - bioluminescence , reactive oxygen species , chemistry , in vivo , biophysics , electron transfer , oxygen , membrane permeability , membrane , biochemistry , biology , photochemistry , microbiology and biotechnology , organic chemistry
A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO 3 H‐APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme‐induced electron transfer (BioLeT) and modulation of cell‐membrane permeability. This probe enables non‐invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes.