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Whole‐Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample
Author(s) -
Han HeeSun,
Cantalupo Paul G.,
Rotem Assaf,
Cockrell Shelley K.,
Carbonnaux Martial,
Pipas James M.,
Weitz David A.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201507047
Subject(s) - metagenomics , genome , computational biology , whole genome sequencing , biology , virus , dna sequencing , sequence analysis , sequence (biology) , virology , genetics , gene
Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop‐based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96 % sequence coverage and more than 99.8 % sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.