Premium
Reversible Supramolecular Surface Attachment of Enzyme–Polymer Conjugates for the Design of Biocatalytic Filtration Membranes
Author(s) -
Moridi Negar,
Corvini Philippe F.X.,
Shahgaldian Patrick
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201507020
Subject(s) - membrane , supramolecular chemistry , conjugate , chemistry , biocatalysis , polymer , filtration (mathematics) , surface modification , immobilized enzyme , chemical engineering , size exclusion chromatography , supramolecular polymers , combinatorial chemistry , macromolecule , polymer chemistry , organic chemistry , enzyme , molecule , reaction mechanism , biochemistry , catalysis , mathematical analysis , statistics , mathematics , engineering
Abstract To be used successfully in continuous reactor systems, enzymes must either be retained using filtration membranes or immobilized on a solid component of the reactor. Whereas the first approach requires large amounts of energy, the second approach is limited by the low temporal stability of enzymes under operational conditions. To circumvent these major stumbling blocks, we have developed a strategy that enables the reversible supramolecular immobilization of bioactive enzyme–polymer conjugates at the surface of filtration membranes. The polymer is produced through a reversible addition–fragmentation transfer method; it contains multiple adamantyl moieties that are used to bind the resulting conjugate at the surface of the membrane which has surface‐immobilized cyclodextrin macrocycles. This supramolecular modification is stable under operational conditions and allows for efficient biocatalysis, and can be reversed by competitive host–guest interactions.