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Organized Aggregation of Porphyrins in Lipid Bilayers for Third Harmonic Generation Microscopy
Author(s) -
Cui Liyang,
Tokarz Danielle,
Cisek Richard,
Ng Kenneth K.,
Wang Fan,
Chen Juan,
Barzda Virginijus,
Zheng Gang
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201506171
Subject(s) - second harmonic generation , microscopy , second harmonic imaging microscopy , fluorescence microscope , fluorescence , nanoparticle , chemistry , biophysics , nanotechnology , colloidal gold , high harmonic generation , materials science , optics , physics , biology , laser
Nonlinear optical microscopy has become a powerful tool for high‐resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo‐damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure‐associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure‐dependent switch between harmonic (OAP‐intact) and fluorescence (OAP‐disrupted) generation enabled real‐time multi‐modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre‐formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging.