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Heat‐Induced Radiolabeling of Nanoparticles for Monocyte Tracking by PET
Author(s) -
Normandin Marc D.,
Yuan Hushan,
Wilks Moses Q.,
Chen Howard H.,
Kinsella Joseph M.,
Cho Hoonsung,
Guehl Nicolas J.,
AbsiHalabi Nader,
Hosseini Seyed Mohammadreza,
El Fakhri Georges,
Sosnovik David E.,
Josephson Lee
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201505525
Subject(s) - chemistry , monocyte , in vitro , spleen , positron emission tomography , pet imaging , immune system , nanoparticle , flow cytometry , biophysics , radiochemistry , lymph , biochemistry , microbiology and biotechnology , nanotechnology , materials science , nuclear medicine , pathology , immunology , medicine , biology
Heat‐induced radiolabeling (HIR) yielded 89 Zr‐Feraheme (FH) nanoparticles (NPs) that were used to determine NP pharmacokinetics (PK) by positron emission tomography (PET). Standard uptake values indicated a fast hepatic uptake that corresponded to blood clearance, and a second, slow uptake process by lymph nodes and spleen. By cytometry, NPs were internalized by circulating monocytes and monocytes in vitro. Using an IV injection of HIR 89 Zr‐FH (rather than in vitro cell labeling), PET/PK provided a view of monocyte trafficking, a key component of the immune response.