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A Small‐Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson’s Disease
Author(s) -
Li Lin,
Zhang ChengWu,
Ge Jingyan,
Qian Linghui,
Chai BingHan,
Zhu Qing,
Lee JunSeok,
Lim KahLeong,
Yao Shao Q.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201504441
Subject(s) - monoamine oxidase b , monoamine oxidase , monoamine oxidase a , proteome , chemistry , biophysics , biochemistry , biology , enzyme
The design of the first dual‐purpose activity‐based probe of monoamine oxidase B (MAO‐B) is reported. This probe is highly selective towards MAO‐B, even at high MAO‐A expression levels, and could sensitively report endogenous MAO‐B activities by both in situ proteome profiling and live‐cell bioimaging. With a built‐in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO‐B imaging probes failed to do thus far: the live‐cell imaging of MAO‐B activities without encountering diffusion problems.