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Dynamic Expression of DNA Complexation with Self‐assembled Biomolecular Clusters
Author(s) -
Bartolami Eline,
Bessin Yannick,
Gervais Virginie,
Dumy Pascal,
Ulrich Sébastien
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201504047
Subject(s) - isothermal titration calorimetry , chemistry , dna , cationic polymerization , covalent bond , biophysics , gel electrophoresis , combinatorial chemistry , biochemistry , organic chemistry , biology
We report herein the implementation of a dynamic covalent chemistry approach to the generation of multivalent clusters for DNA recognition. We show that biomolecular clusters can be expressed in situ by a programmed self‐assembly process using chemoselective ligations. The cationic clusters are shown, by fluorescence displacement assay, gel electrophoresis and isothermal titration calorimetry, to effectively complex DNA through multivalent interactions. The reversibility of the ligation was exploited to demonstrate that template effects occur, whereby DNA imposes component selection in order to favor the most active DNA‐binding clusters. Furthermore, we show that a chemical effector can be used to trigger DNA release through component exchange reactions.