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Prometabolites of 5‐Diphospho‐ myo ‐inositol Pentakisphosphate
Author(s) -
Pavlovic Igor,
Thakor Divyeshsinh T.,
Bigler Laurent,
Wilson Miranda S. C.,
Laha Debabrata,
Schaaf Gabriel,
Saiardi Adolfo,
Jessen Henning J.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201503094
Subject(s) - inositol , second messenger system , chemistry , biochemistry , inositol phosphate , enzyme , membrane , metabolism , biophysics , microbiology and biotechnology , biology , receptor
Diphospho‐ myo ‐inositol phosphates (PP‐InsP y ) are an important class of cellular messengers. Thus far, no method for the transport of PP‐InsP y into living cells is available. Owing to their high negative charge density, PP‐InsP y will not cross the cell membrane. A strategy to circumvent this issue involves the generation of precursors in which the negative charges are masked with biolabile groups. A PP‐InsP y prometabolite would require twelve to thirteen biolabile groups, which need to be cleaved by cellular enzymes to release the parent molecules. Such densely modified prometabolites of phosphate esters and anhydrides have never been reported to date. This study discloses the synthesis of such agents and an analysis of their metabolism in tissue homogenates by gel electrophoresis. The acetoxybenzyl‐protected system is capable of releasing 5‐PP‐InsP 5 in mammalian cell/tissue homogenates within a few minutes and can be used to release 5‐PP‐InsP 5 inside cells. These molecules will serve as a platform for the development of fundamental tools required to study PP‐InsP y physiology.