Premium
Boronate‐Affinity Glycan‐Oriented Surface Imprinting: A New Strategy to Mimic Lectins for the Recognition of an Intact Glycoprotein and Its Characteristic Fragments
Author(s) -
Bie Zijun,
Chen Yang,
Ye Jin,
Wang Shuangshou,
Liu Zhen
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201503066
Subject(s) - glycomics , glycan , molecularly imprinted polymer , lectin , chemistry , molecular imprinting , molecular recognition , imprinting (psychology) , glycoprotein , combinatorial chemistry , biochemistry , nanotechnology , selectivity , molecule , organic chemistry , materials science , gene , catalysis
Lectins possess unique binding properties and are of particular value in molecular recognition. However, lectins suffer from several disadvantages, such as being hard to prepare and showing poor storage stability. Boronate‐affinity glycan‐oriented surface imprinting was developed as a new strategy for the preparation of lectin‐like molecularly imprinted polymers (MIPs). The prepared MIPs could specifically recognize an intact glycoprotein and its characteristic fragments, even within a complex sample matrix. Glycan‐imprinted MIPs could thus prove to be powerful tools for important applications such as proteomics, glycomics, and diagnostics.