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Reversibly Triggered Protein–Ligand Assemblies in Giant Vesicles
Author(s) -
Peters Ruud J. R. W.,
Nijemeisland Marlies,
van Hest Jan C. M.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201502920
Subject(s) - vesicle , chemistry , ligand (biochemistry) , molecule , biophysics , small molecule , membrane , biochemistry , receptor , organic chemistry , biology
External small‐molecule triggers were used to reversibly control dynamic protein–ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small‐molecule substrates, thereby modulating the pH‐sensitive interaction between a Ni‐NTA ligand on the vesicle membrane and an oligohistidine‐tagged protein in the lumen. By alternating the small‐molecule substrates the interaction could be reversed.