Premium
Photoelectrocyclization as an Activation Mechanism for Organelle‐Specific Live‐Cell Imaging Probes
Author(s) -
Tran Mai N.,
Chenoweth David M.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201502403
Subject(s) - organelle , live cell imaging , chemistry , cell , biophysics , mechanism (biology) , fluorescence , fluorescence lifetime imaging microscopy , stokes shift , molecular imaging , nanotechnology , materials science , biochemistry , biology , physics , microbiology and biotechnology , quantum mechanics , in vivo
Abstract Photoactivatable fluorophores are useful tools in live‐cell imaging owing to their potential for precise spatial and temporal control. In this report, a new photoactivatable organelle‐specific live‐cell imaging probe based on a 6π electrocyclization/oxidation mechanism is described. It is shown that this new probe is water‐soluble, non‐cytotoxic, cell‐permeable, and useful for mitochondrial imaging. The probe displays large Stokes shifts in both pre‐activated and activated forms, allowing simultaneous use with common dyes and fluorescent proteins. Sequential single‐cell activation experiments in dense cellular environments demonstrate high spatial precision and utility in single‐ or multi‐cell labeling experiments.