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Programmed Synthesis by Stimuli‐Responsive DNAzyme‐Modified Mesoporous SiO 2 Nanoparticles
Author(s) -
Balogh Dora,
Aleman Garcia Miguel Angel,
Albada H. Bauke,
Willner Itamar
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201501777
Subject(s) - deoxyribozyme , chemistry , mesoporous material , nanoparticle , conjugated system , fluorescence , combinatorial chemistry , nanotechnology , catalysis , materials science , dna , organic chemistry , biochemistry , polymer , physics , quantum mechanics
DNAzyme‐capped mesoporous SiO 2 nanoparticles (MP SiO 2 NPs) are applied as stimuli‐responsive containers for programmed synthesis. Three types of MP SiO 2 NPs are prepared by loading the NPs with Cy3‐DBCO (DBCO=dibenzocyclooctyl), Cy5‐N 3 , and Cy7‐N 3 , and capping the NP containers with the Mg 2+ , Zn 2+ , and histidine‐dependent DNAzyme sequences, respectively. In the presence of Mg 2+ and Zn 2+ ions as triggers, the respective DNAzyme‐capped NPs are unlocked, leading to the “click” reaction product Cy3‐Cy5. In turn, in the presence of Mg 2+ ions and histidine as triggers the second set of DNAzyme‐capped NPs is unlocked leading to the Cy3‐Cy7 conjugated product. The unloading of the respective NPs and the time‐dependent formation of the products are followed by fluorescence spectroscopy (FRET). A detailed kinetic model for the formation of the different products is formulated and it correlates nicely with the experimental results.