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Hyperbranched Hybridization Chain Reaction for Triggered Signal Amplification and Concatenated Logic Circuits
Author(s) -
Bi Sai,
Chen Min,
Jia Xiaoqiang,
Dong Ying,
Wang Zonghua
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201501457
Subject(s) - keypad , chain reaction , lock (firearm) , dna , computer science , branch migration , electronic circuit , logic gate , cascade , chemistry , nanotechnology , computer hardware , materials science , algorithm , engineering , dna repair , electrical engineering , biochemistry , mechanical engineering , photochemistry , chromatography , holliday junction
A hyper‐branched hybridization chain reaction (HB‐HCR) is presented herein, which consists of only six species that can metastably coexist until the introduction of an initiator DNA to trigger a cascade of hybridization events, leading to the self‐sustained assembly of hyper‐branched and nicked double‐stranded DNA structures. The system can readily achieve ultrasensitive detection of target DNA. Moreover, the HB‐HCR principle is successfully applied to construct three‐input concatenated logic circuits with excellent specificity and extended to design a security‐mimicking keypad lock system. Significantly, the HB‐HCR‐based keypad lock can alarm immediately if the “password” is incorrect. Overall, the proposed HB‐HCR with high amplification efficiency is simple, homogeneous, fast, robust, and low‐cost, and holds great promise in the development of biosensing, in the programmable assembly of DNA architectures, and in molecular logic operations.