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Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation
Author(s) -
Pawlak Joanna B.,
Gential Geoffroy P. P.,
Ruckwardt Tracy J.,
Bremmers Jessica S.,
Meeuwenoord Nico J.,
Ossendorp Ferry A.,
Overkleeft Herman S.,
Filippov Dmitri V.,
van Kasteren Sander I.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201500301
Subject(s) - bioorthogonal chemistry , antigen , chemistry , antigen processing , cross presentation , antigen presentation , cell , combinatorial chemistry , biochemistry , microbiology and biotechnology , click chemistry , biology , cytotoxic t cell , immunology , in vitro
The activation of CD8 + T‐cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC‐I complexes and presented to the T‐cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated—antigens that are not recognized by their cognate T‐cell unless they are deprotected on the cell using a Staudinger reduction.