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Folding and Imaging of DNA Nanostructures in Anhydrous and Hydrated Deep‐Eutectic Solvents
Author(s) -
Gállego Isaac,
Grover Martha A.,
Hud Nicholas V.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201412354
Subject(s) - aqueous solution , deep eutectic solvent , nanostructure , solvent , choline chloride , dna origami , folding (dsp implementation) , chemistry , eutectic system , nanotechnology , anhydrous , chemical engineering , materials science , crystallography , organic chemistry , microstructure , engineering , electrical engineering
There is great interest in DNA nanotechnology, but its use has been limited to aqueous or substantially hydrated media. The first assembly of a DNA nanostructure in a water‐free solvent, namely a low‐volatility biocompatible deep‐eutectic solvent composed of a 4:1 mixture of glycerol and choline chloride (glycholine), is now described. Glycholine allows for the folding of a two‐dimensional DNA origami at 20 °C in six days, whereas in hydrated glycholine, folding is accelerated (≤3 h). Moreover, a three‐dimensional DNA origami and a DNA tail system can be folded in hydrated glycholine under isothermal conditions. Glycholine apparently reduces the kinetic traps encountered during folding in aqueous solvent. Furthermore, folded structures can be transferred between aqueous solvent and glycholine. It is anticipated that glycholine and similar solvents will allow for the creation of functional DNA structures of greater complexity by providing a milieu with tunable properties that can be optimized for a range of applications and nanostructures.