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Real‐Time Monitoring of Phosphorylation Kinetics with Self‐Assembled Nano‐oscillators
Author(s) -
Fang Yimin,
Chen Shan,
Wang Wei,
Shan Xiaonan,
Tao gjian
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201411040
Subject(s) - phosphorylation , nanoparticle , nanotechnology , oscillation (cell signaling) , kinetics , colloidal gold , nano , plasmon , chemistry , linker , materials science , electric field , optoelectronics , computer science , physics , biochemistry , quantum mechanics , composite material , operating system
Abstract Phosphorylation is a post‐translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label‐free detection of phosphorylation is reported in real time with self‐assembled nano‐oscillators. Each nano‐oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano‐oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano‐oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.