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EPR‐Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules
Author(s) -
Abdullin Dinar,
Florin Nicole,
Hagelueken Gregor,
Schiemann Olav
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201410396
Subject(s) - electron paramagnetic resonance , biomolecule , metal ions in aqueous solution , paramagnetism , ion , pulsed epr , azurin , chemistry , metal , trilateration , copper , nanotechnology , crystallography , materials science , nuclear magnetic resonance , magnetic resonance imaging , physics , spin echo , condensed matter physics , organic chemistry , medicine , radiology , quantum mechanics , node (physics)
Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three‐dimensional fold of such biomolecules is therefore an important goal in understanding structure–function relationships. A trilateration approach for the localization of metal ions by means of long‐range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu 2+ center of azurin, and factors affecting the precision of the method are discussed.