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Nanoparticle‐Mediated Binning and Profiling of Heterogeneous Circulating Tumor Cell Subpopulations
Author(s) -
Mohamadi Reza M.,
Besant Justin D.,
Mepham Adam,
Green Brenda,
Mahmoudian Laili,
Gibbs Thaddeus,
Ivanov Ivaylo,
Malvea Anahita,
Stojcic Jessica,
Allan Alison L.,
Lowes Lori E.,
Sargent Edward H.,
Nam Robert K.,
Kelley Shana O.
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201409376
Subject(s) - circulating tumor cell , phenotype , population , mesenchymal stem cell , computational biology , cancer research , biology , cancer , medicine , metastasis , microbiology and biotechnology , gene , genetics , environmental health
Abstract The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters blood‐borne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial‐to‐mesenchymal transition (EMT).