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Differential Sensing of MAP Kinases Using SOX‐Peptides
Author(s) -
ZamoraOlivares Diana,
Kaoud Tamer S.,
Jose Jiney,
Ellington Andrew,
Dalby Kevin N.,
Anslyn Eric V.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201408256
Subject(s) - kinase , mapk/erk pathway , gene isoform , chemometrics , mitogen activated protein kinase , chemistry , computational biology , biochemistry , chromatography , biology , gene
Five SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross‐reactivity among the peptides, and some unexpected surprises.