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HyperBIRD: A Sensitivity‐Enhanced Approach to Collecting Homonuclear‐Decoupled Proton NMR Spectra
Author(s) -
Donovan Kevin J.,
Frydman Lucio
Publication year - 2015
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201407869
Subject(s) - homonuclear molecule , heteronuclear molecule , spectral line , chemistry , nmr spectra database , proton , polarization (electrochemistry) , two dimensional nuclear magnetic resonance spectroscopy , spins , molecule , nuclear magnetic resonance , analytical chemistry (journal) , chemical physics , molecular physics , stereochemistry , physics , nuclear physics , organic chemistry , astronomy , condensed matter physics
Samples prepared following dissolution dynamic nuclear polarization (DNP) enable the detection of NMR spectra from low‐γ nuclei with outstanding sensitivity, yet have limited use for the enhancement of abundant species like 1 H nuclei. Small‐ and intermediate‐sized molecules, however, show strong heteronuclear cross‐relaxation effects: spontaneous processes with an inherent isotopic selectivity, whereby only the 13 C‐bonded protons receive a polarization enhancement. These effects are here combined with a recently developed method that delivers homonuclear‐decoupled 1 H spectra in natural abundance samples based on heteronuclear couplings to these same, 13 C‐bonded nuclei. This results in the HyperBIRD methodology; a single‐shot combination of these two effects that can simultaneously simplify and resolve complex, congested 1 H NMR spectra with many overlapping spin multiplets, while achieving 50–100 times sensitivity enhancements over conventional thermal counterparts.