Premium
Synthesis of and Specific Antibody Generation for Glycopeptides with Arginine N ‐GlcNAcylation
Author(s) -
Pan Man,
Li Shan,
Li Xiang,
Shao Feng,
Liu Lei,
Hu HongGang
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201407824
Subject(s) - glycopeptide , glycosylation , arginine , glycoprotein , biochemistry , antibody , chemistry , computational biology , lysine , sequence (biology) , peptide , biology , amino acid , immunology , antibiotics
Abstract As a unique and unappreciated protein posttranslational modification, arginine N ‐glycosylation was recently discovered to play an important role in the process that bacteria counteract host defenses. To provide chemical tools for further proteomic and biochemical studies on arginine N ‐glycosylation, we report the first general strategy for a rapid and cost‐effective synthesis of glycopeptides carrying single or multiple arginine N ‐GlcNAcyl groups. These glycopeptides were successfully utilized to generate the first antibodies that can specifically recognize arginine N ‐GlcNAcylated peptides or proteins in a sequence‐independent manner.