Synthesis of and Specific Antibody Generation for Glycopeptides with Arginine N ‐GlcNAcylation

As a unique and unappreciated protein posttranslational modification, arginine N ‐glycosylation was recently discovered to play an important role in the process that bacteria counteract host defenses. To provide chemical tools for further proteomic and biochemical studies on arginine N ‐glycosylation, we report the first general strategy for a rapid and cost‐effective synthesis of glycopeptides carrying single or multiple arginine N ‐GlcNAcyl groups. These glycopeptides were successfully utilized to generate the first antibodies that can specifically recognize arginine N ‐GlcNAcylated peptides or proteins in a sequence‐independent manner.