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Reversible Ca 2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme
Author(s) -
Zhang Chunqiu,
Pan Tiezheng,
Salesse Christian,
Zhang Dongmei,
Miao Lu,
Wang Liang,
Gao Yuzhou,
Xu Jiayun,
Dong Zeyuan,
Luo Quan,
Liu Junqiu
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201407135
Subject(s) - chemistry , antioxidant , glutathione , glutathione peroxidase , allosteric regulation , selenium , biophysics , biochemistry , enzyme , organic chemistry , biology
A Ca 2+ ‐responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium‐containing recoverin exhibits high GPx activity for the catalyzed reduction of H 2 O 2 by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca 2+ ‐induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications.