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Sequence and Chiral Selectivity of Drug–DNA Interactions Revealed by Force Spectroscopy
Author(s) -
Hu Qiongzheng,
Xu Shoujun
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201407093
Subject(s) - force spectroscopy , chemistry , molecule , dna , selectivity , nuclear magnetic resonance spectroscopy , chirality (physics) , molecular binding , stereochemistry , spectroscopy , crystallography , biochemistry , organic chemistry , physics , nambu–jona lasinio model , chiral symmetry breaking , quantum mechanics , quark , catalysis
Abstract Differential binding force has been used to precisely characterize the mechanical effect of a drug molecule binding to a DNA duplex. The high‐resolution binding forces measured by the force‐induced remnant magnetization spectroscopy (FIRMS) enable the binding behavior of drug molecules with different chirality and DNA of various sequences to be distinguished. The sequence specificity of Hg 2+ and daunomycin was revealed by force spectroscopy for the first time, and the results are consistent with those obtained by other techniques. Furthermore, the two isomers of d,l ‐tetrahydropalmatine showed selectivity for two different DNA sequences. One particular useful feature of this approach is that the small molecules under study do not require any labels.