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Bright, Highly Water‐Soluble Triazacyclononane Europium Complexes To Detect Ligand Binding with Time‐Resolved FRET Microscopy
Author(s) -
Delbianco Martina,
Sadovnikova Victoria,
Bourrier Emmanuel,
Mathis Gérard,
Lamarque Laurent,
Zwier Jurriaan M.,
Parker David
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201406632
Subject(s) - förster resonance energy transfer , europium , chemistry , ligand (biochemistry) , fluorescence , luminescence , fluorescence microscope , confocal microscopy , membrane , sulfonic acid , microscopy , biophysics , photochemistry , receptor , polymer chemistry , organic chemistry , biochemistry , materials science , ion , physics , optoelectronics , quantum mechanics , optics , biology , microbiology and biotechnology
Luminescent europium complexes are used in a broad range of applications as a result of their particular emissive properties. The synthesis and application of bright, highly water‐soluble, and negatively charged sulfonic‐ or carboxylic acid derivatives of para ‐substituted aryl–alkynyl triazacyclononane complexes are described. Introduction of the charged solubilizing moieties suppresses cellular uptake or adsorption to living cells making them applicable for labeling and performing assays on membrane receptors. These europium complexes are applied to monitor fluorescent ligand binding on cell‐surface proteins with time‐resolved Förster resonance energy transfer (TR‐FRET) assays in plate‐based format and using TR‐FRET microscopy.