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Super‐Resolution Imaging of Plasma Membrane Glycans
Author(s) -
Letschert Sebastian,
Göhler Antonia,
Franke Christian,
BertleffZieschang Nadja,
Memmel Elisabeth,
Doose Sören,
Seibel Jürgen,
Sauer Markus
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201406045
Subject(s) - bioorthogonal chemistry , glycan , chemistry , glycoconjugate , glycosylation , membrane , sialic acid , biophysics , biochemistry , fluorescence , cell membrane , click chemistry , glycoprotein , combinatorial chemistry , biology , physics , quantum mechanics
Much of the physiology of cells is controlled by the spatial organization of the plasma membrane and the glycosylation patterns of its components, however, studying the distribution, size, and composition of these components remains challenging. A bioorthogonal chemical reporter strategy was used for the efficient and specific labeling of membrane‐associated glycoconjugates with modified monosaccharide precursors and organic fluorophores. Super‐resolution fluorescence imaging was used to visualize plasma membrane glycans with single‐molecule sensitivity. Our results demonstrate a homogeneous distribution of N ‐acetylmannosamine (ManNAc)‐, N ‐acetylgalactosamine (GalNAc)‐, and O‐linked N ‐acetylglucosamine (O‐GlcNAc)‐modified plasma membrane proteins in different cell lines with densities of several million glycans on each cell surface.