Premium
RNA Interference Controlled by Light of Variable Wavelength
Author(s) -
Meyer Andreas,
Mokhir Andriy
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201405885
Subject(s) - photosensitizer , chemistry , small interfering rna , photochemistry , biophysics , rna , biochemistry , gene , biology
Known molecular, “caged” siRNAs are activated by UV light. Since the light of this type is toxic to cells, the uncaging can cause undesired side effects. A modular, molecular system for designing siRNAs is reported, which can be activated by non‐toxic light in live cells. For example, siRNAs responsive to green and red light are described. The uncaging is mediated by 1 O 2 photogenerated on a photosensitizer, which is attached to the 3′‐terminus of the lagging strand. The 5′‐terminus of the guide strand is alkylated (“caged”) with a 9‐anthracenyl residue. The latter fragment reacts with the 1 O 2 with formation of the free (uncaged) 5′‐OH terminus. Simultaneously with the uncaging the photosensitizer is bleached and no more 1 O 2 is generated after this process is completed. The photoactivation of the siRNAs described here is not toxic to cells.