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Stable Small Quantum Dots for Synaptic Receptor Tracking on Live Neurons
Author(s) -
Cai En,
Ge Pinghua,
Lee Sang Hak,
Jeyifous Okunola,
Wang Yong,
Liu Yanxin,
Wilson Katie M.,
Lim Sung Jun,
Baird Michelle A.,
Stone John E.,
Lee Kwan Young,
Davidson Michael W.,
Chung Hee Jung,
Schulten Klaus,
Smith Andrew M.,
Green William N.,
Selvin Paul R.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201405735
Subject(s) - quantum dot , postsynaptic density , nanotechnology , postsynaptic potential , materials science , ampa receptor , receptor , chemistry , glutamate receptor , biochemistry
We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super‐resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.