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Tautomerism in Neutral Histidine
Author(s) -
Bermúdez Celina,
Mata Santiago,
Cabezas Carlos,
Alonso José L.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201405347
Subject(s) - tautomer , histidine , hyperfine structure , chemistry , quadrupole , side chain , computational chemistry , stereochemistry , crystallography , amino acid , atomic physics , organic chemistry , physics , biochemistry , polymer
Histidine is an important natural amino acid, involved in many relevant biological processes, which, because of its physical properties, proved difficult to characterize experimentally in its neutral form. In this work, neutral histidine has been generated in the gas phase by laser ablation of solid samples and its N ε H tautomeric form unraveled through its rotational spectrum. The quadrupole hyperfine structure, arising from the existing three 14 N nuclei, constituted a site‐specifically probe for revealing the tautomeric form as well as the side chain configuration of this proteogenic amino acid.