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A Method for Evaluating the Level of Soluble β‐Amyloid (1–40/1–42) in Alzheimer’s Disease Based on the Binding of Gelsolin to β‐Amyloid Peptides
Author(s) -
Yu Yanyan,
Zhang Lin,
Li Chenglin,
Sun Xiaoyu,
Tang Daoquan,
Shi Guoyue
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201405001
Subject(s) - gelsolin , amyloid (mycology) , cerebrospinal fluid , alzheimer's disease , β amyloid , chemistry , biochemistry , peptide , antibody , amyloid β , disease , medicine , immunology , actin , inorganic chemistry
In the present work, a new electrochemical strategy for the sensitive and specific detection of soluble β‐amyloid Aβ (1–40/1–42) peptides in a rat model of Alzheimer’s disease (AD) is described. In contrast to previous antibody‐based methods, β‐amyloid (1–40/1–42) was quantified based on its binding to gelsolin, a secretory protein present in the cerebrospinal fluid (CSF) and plasma. The level of soluble β‐amyloid peptides in the CSF and various brain regions were found with this method to be lower in rats with AD than in normal rats.