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Imidazole‐Based Small Molecules that Promote Neurogenesis in Pluripotent Cells
Author(s) -
Kim GunHee,
Halder Debasish,
Park Jinhong,
Namkung Wan,
Shin Injae
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201404871
Subject(s) - neurogenesis , p19 cell , retinoic acid , induced pluripotent stem cell , microbiology and biotechnology , chemistry , glutamate receptor , cellular differentiation , cell , embryonic stem cell , biology , neuroscience , biochemistry , receptor , gene
Reported herein are two imidazole‐based small molecules, termed neurodazine (Nz) and neurodazole (Nzl), which induce neuronal differentiation of pluripotent P19 cells. Their ability to induce neurogenesis of P19 cells is comparable to that of retinoic acid. However, Nz and Nzl were found to be more selective neurogenesis inducers than retinoic acid owing to their unique ability to suppress astrocyte differentiation of P19 cells. Our results also show that Nz and Nzl promote production of physiologically active neurons because P19‐cell‐derived neurons induced by these substances have functional glutamate responsiveness. The present study suggests that Nz and Nzl could serve as important chemical tools to induce formation of specific populations of neuronal cell types from pluripotent cells.