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Nanoelectrode for Amperometric Monitoring of Individual Vesicular Exocytosis Inside Single Synapses
Author(s) -
Li YuTao,
Zhang ShuHui,
Wang Li,
Xiao RongRong,
Liu Wei,
Zhang XinWei,
Zhou Zhuan,
Amatore Christian,
Huang WeiHua
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201404744
Subject(s) - exocytosis , amperometry , neurotransmission , synapse , nanotechnology , neurotransmitter , chemistry , synaptic vesicle , neuroscience , vesicle , electrochemistry , materials science , biology , membrane , electrode , biochemistry , receptor , central nervous system
Chemical neurotransmission occurs at chemical synapses and endocrine glands, but up to now there was no means for direct monitoring of neurotransmitter exocytosis fluxes and their precise kinetics from inside an individual synapse. The fabrication of a novel finite conical nanoelectrode is reported perfectly suited in size and electrochemical properties for probing amperometrically inside what appears to be single synapses and monitoring individual vesicular exocytotic events in real time. This allowed obtaining direct and important physiological evidences which may yield important and new insights into the nature of synaptic communications.