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Immunological Multimetal Deposition for Rapid Visualization of Sweat Fingerprints
Author(s) -
He Yayun,
Xu Linru,
Zhu Yu,
Wei Qianhui,
Zhang Meiqin,
Su Bin
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201404416
Subject(s) - chemistry , sweat , colloidal gold , immunoassay , deposition (geology) , chromatography , visualization , nanotechnology , nanoparticle , materials science , antibody , computer science , biology , paleontology , artificial intelligence , sediment , immunology
A simple method termed immunological multimetal deposition (iMMD) was developed for rapid visualization of sweat fingerprints with bare eyes, by combining the conventional MMD with the immunoassay technique. In this approach, antibody‐conjugated gold nanoparticles (AuNPs) were used to specifically interact with the corresponding antigens in the fingerprint residue. The AuNPs serve as the nucleation sites for autometallographic deposition of silver particles from the silver staining solution, generating a dark ridge pattern for visual detection. Using fingerprints inked with human immunoglobulin G (hIgG), we obtained the optimal formulation of iMMD, which was then successfully applied to visualize sweat fingerprints through the detection of two secreted polypeptides, epidermal growth factor and lysozyme. In comparison with the conventional MMD, iMMD is faster and can provide additional information than just identification. Moreover, iMMD is facile and does not need expensive instruments.