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Bio‐reduction of Redox‐Sensitive Albumin Conjugates in FcRn‐Expressing Cells
Author(s) -
Brülisauer Lorine,
Valentino Gina,
Morinaga Sakura,
Cam Kübra,
Thostrup Bukrinski Jens,
Gauthier Marc A.,
Leroux JeanChristophe
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201404238
Subject(s) - conjugate , albumin , chemistry , neonatal fc receptor , prodrug , redox , serum albumin , biochemistry , bovine serum albumin , human albumin , cell culture , microbiology and biotechnology , biophysics , immunoglobulin g , biology , immunology , antibody , mathematical analysis , genetics , mathematics , organic chemistry
Disulfide‐containing IgG‐, Fc‐, or albumin‐based prodrugs that rely on FcRn‐trafficking by endothelial cells for prolonged circulation in the body might be hampered by premature bio‐reduction processes during FcRn‐mediated recycling events. A detailed bio‐reduction analysis of redox‐sensitive albumin conjugates in two FcRn‐expressing cell lines has been performed. The obtained results indicate that the FcRn‐mediated recycling pathway is not (or is only poorly) bio‐reducing.