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Single‐Molecule Mechanochemical Sensing Using DNA Origami Nanostructures
Author(s) -
Koirala Deepak,
Shrestha Prakash,
Emura Tomoko,
Hidaka Kumi,
Mandal Shankar,
Endo Masayuki,
Sugiyama Hiroshi,
Mao Hanbin
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201404043
Subject(s) - dna origami , nanotechnology , nanostructure , molecule , tweezers , dna , multiplex , materials science , dna nanotechnology , chemistry , bioinformatics , biology , biochemistry , organic chemistry
While single‐molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single‐molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7‐tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 p M platelet‐derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single‐molecule sensing with improved throughput.