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Dynamics of Intact Immunoglobulin G Explored by Drift‐Tube Ion‐Mobility Mass Spectrometry and Molecular Modeling
Author(s) -
Pacholarz Kamila J.,
Porrini Massimiliano,
Garlish Rachel A.,
Burnley Rebecca J.,
Taylor Richard J.,
Henry Alistair J.,
Barran Perdita E.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201402863
Subject(s) - ion mobility spectrometry , subclass , chemistry , mass spectrometry , ion , molecular dynamics , drift tube , range (aeronautics) , analytical chemistry (journal) , chemical physics , antibody , chromatography , materials science , computational chemistry , organic chemistry , immunology , composite material , biology
Collision cross‐sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift‐tube ion‐mobility mass spectrometry. Intact antibodies and Fc‐hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.