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Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
Author(s) -
Vaníková Zuzana,
Hocek Michal
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201402370
Subject(s) - restriction enzyme , polymerase , cleavage (geology) , chemistry , dna polymerase , nucleic acid , dna , microbiology and biotechnology , deoxyuridine , biochemistry , biology , paleontology , fracture (geology)
5‐[(2‐Nitrobenzyl)oxymethyl]‐2′‐deoxyuridine 5′‐ O ‐triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5‐hydroxymethyluracil‐modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.