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Threshold Sensing through a Synthetic Enzymatic Reaction–Diffusion Network
Author(s) -
Semenov Sergey N.,
Markvoort Albert J.,
de Greef Tom F. A.,
Huck Wilhelm T. S.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201402327
Subject(s) - autocatalysis , biological system , reaction–diffusion system , enzyme , nanotechnology , diffusion , synthetic biology , chemistry , reaction conditions , enzyme catalysis , chemical reaction , lithography , combinatorial chemistry , materials science , computer science , organic chemistry , catalysis , computational biology , physics , biology , thermodynamics , optoelectronics
A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.