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Construction of a Live‐Attenuated HIV‐1 Vaccine through Genetic Code Expansion
Author(s) -
Wang Nanxi,
Li Yue,
Niu Wei,
Sun Ming,
Cerny Ronald,
Li Qingsheng,
Guo Jiantao
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201402092
Subject(s) - virology , human immunodeficiency virus (hiv) , virulence , attenuated vaccine , viral replication , replication (statistics) , genome , virus , pandemic , biology , genetic code , computational biology , genetics , medicine , gene , covid-19 , disease , infectious disease (medical specialty) , pathology
A safe and effective vaccine against human immunodeficiency virus type 1 (HIV‐1) is urgently needed to combat the worldwide AIDS pandemic, but still remains elusive. The fact that uncontrolled replication of an attenuated vaccine can lead to regaining of its virulence creates safety concerns precluding many vaccines from clinical application. We introduce a novel approach to control HIV‐1 replication, which entails the manipulation of essential HIV‐1 protein biosynthesis through unnatural amino acid (UAA*)‐mediated suppression of genome‐encoded blank codon. We successfully demonstrate that HIV‐1 replication can be precisely turned on and off in vitro.